Development of a rapid and sensitive determination method of carbohydrates and their related compounds by capillary electrophoresis with amperometric detection at a copper microelectrode.

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  • GOTO Masashi
    Department of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University
  • TANAKA Kazuya
    Department of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University
  • ESAKA Yukihiro
    Department of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University
  • UNO Bunji
    Department of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University

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Other Title
  • 銅電極を用いる電気化学検出キャピラリー電気泳動による糖類及びその関連物質の迅速高感度分析法の開発
  • ドウ デンキョク オ モチイル デンキ カガク ケンシュツ キャピラリー デン

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Abstract

Capillary zone electrophoresis was employed to rapidly separate sugars, sugar alcohol, and sugar acids according to their electrophoretic mobilities in a strong alkaline solution containing a cationic surfactant, cetyltrimethylammonium chloride. Sample zones were monitored electrochemically using amperometric detection at a constant potential of 0.7 V (vs. Ag/AgCl) with a copper-disk microelectrode (30 μm in diameter). The copper microelectrode in strong basic solutions with a cationic surfactant showed about two-times the electrocatalytic oxidation currents for carbohydrates and related compounds compared to those without a cationic surfactant. A sample mixture of carbohydrates and related compounds was separated in less than 15 min, being one third compared with that of the conventional method. The detection limits for the samples studied were about 2.5 f mol (S/N=3), being about one twentieth compared with that of the conventional method.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 46 (2), 95-100, 1997

    The Japan Society for Analytical Chemistry

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