Ammonia responsiveness of a magnesium phosphate glass membrane containing silver oxide and its application to enzyme electrode sensors.

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  • 酸化銀を含むリン酸マグネシウムガラス膜のアンモニア応答性とその酵素電極への応用
  • サンカ ギン オ フクム リンサン マグネシウム ガラス マク ノ アンモニア

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Abstract

An alkali-free magnesium phosphate glass membrane containing silver oxide can be applied to the sensor for ammonia liberated from the enzyme reaction with L-aminoacid oxidase. This membrane responded to the large majority of monovalent anions and ammonia but not to acetate and hydroxide ions. The procedure for the immobilization of enzyme was accomplished by covalent bonding on the nylon net with L-aminoacid oxidase-bovine serum albumin-glutaraldehyde system. The probe was carefully made by overlapping the immobilized enzyme membrane on the glass sensing membrane. The potential response of the probe for some L-aminoacids shows a linear relationship between 10-2 and 5×10-4 M at a pH of about 910 at 40°C. It can be used repeatedly for about a hundred runs, and is stable for at least two months. The determination of various L-aminoacids, such as L-cysteine, L-arginine, L-lysine and L-histidine, was established as follows: 10 ml each of 0.05 M K2SO4 and 0.01 M KOH in a 100 ml beaker with suitable amounts of L-aminoacid sample added were diluted to 100 ml, and then heated at 40°C. When the probe was immersed in the solution, a stable equilibrium potential was attained after about one minute. The relative standard deviation for five determinations of 10-4 M L-histidine was within 3 %.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 38 (1), 29-33, 1989

    The Japan Society for Analytical Chemistry

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