液体クロマトグラフィー : 大気圧化学イオン化質量分析法による血清デヒドロエピアンドロステロンサルフェートの定量(<特集>質量分析法の応用技術)
書誌事項
- タイトル別名
-
- Quantification of serum dehydroepiandrosterone sulfate by liquid chromatography/atmospheric pressure chemical ionization mass spectrometry.
- 液体クロマトグラフィー/大気圧化学イオン化質量分析法による血清デヒドロエピアンドロステロンサルフェートの定量
- エキタイ クロマトグラフィータイキアツ カガク イオンカ シツリョウ ブンセキ
この論文をさがす
抄録
Liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (LC/APCI-MS) combined with a column switching technique was applied to the quantification of serum dehydroepiandrosterone sulfate (DHEA-S). Human serum samples were directly injected into the sample preparation column (TSK precolumn BSA-ODS), and then DHEA-S was separated by an analytical column of Inertsil ODS-2. DHEA-S was detected on-line by a Hitachi M 1000 mass spectrometer equipped with an APCI interface. For the detection of DHEA-S, the drift, focus, and multiplier voltages were set at -70 V, -120 V and 2.4 kV, and the vaporizer and the desolvation temperatures were set at 280 and 399°C, respectively. The applied potential for the needle electrode was -10 μA. The detection limit of DHEA-S was 10 ng on-column with a signal-to-noise ratio of 3. The calibration curve was linear over the range of 50300 ng on-column. The proposed method was successfully developed for the determination of DHEA-S in human serum.
収録刊行物
-
- 分析化学
-
分析化学 45 (6), 517-522, 1996
公益社団法人 日本分析化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679028679936
-
- NII論文ID
- 110002907047
-
- NII書誌ID
- AN00222633
-
- NDL書誌ID
- 3966873
-
- ISSN
- 05251931
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可