Enantiomer separation and complexation equilibrium of dansyl-D,L-amino acids with .BETA.-cyclodextrin in capillary electrophoresis.
-
- ISHIGURO Gaku
- Department of Environmental Sciences, Faculty of Science, Ibaraki University
-
- IMURA Hisanori
- Department of Environmental Sciences, Faculty of Science, Ibaraki University
Bibliographic Information
- Other Title
-
- β‐シクロデキストリンを用いるキャピラリー電気泳動によるダンシル‐D,L‐アミノ酸の光学異性体分離とその化学平衡
- β-シクロデキストリンを用いるキャピラリー電気泳動によるダンシル-D,L-アミノ酸の光学異性体分離とその化学平衡
- ベータ シクロデキストリン オ モチイル キャピラリー デンキ エイドウ ニ ヨル ダンシル D L アミノサン ノ コウガク イセイタイ ブンリ ト ソノ カガク ヘイコウ
Search this article
Abstract
The enantiomer separation of 5-dimethylamino-1-naphthalene sulfonyl (Dns) derivatives of D,L-phenylalanine and D,L-isoleucine has been studied by capillary zone electrophoresis (CZE) mediated with β-cyclodextrin (β-CD) as a chiral selector. The baseline separation of the respective D and L isomers was observed at the pH region around each isoelectric point, i.e., pH 2.5-4.5, except for the isoelectric point, itself. To understand such phenomena, the solution equilibria of the acid dissociation of Dns-D,L-Phe (designated by HA for the neutral species) and the formation of inclusion complexes with β-CD were studied by CZE at 25°C and an ionic strength of 0.005 M. The electrophoretic mobility (μe) of Dns-D,L-Phe was measured as a function of the pH and of the β-CD concentration, and analyzed by a three-dimensional non-linear least-squares method (SigmaPlot®). The acid-dissociation constants of H2A+ and HA±, the formation constant of the inclusion complexes, HA± · CD and A- · CD, and the electric mobilities of H2A+, A-, and A- · CD were determined and analyzed. The high enantiomer-separation ability observed around the isoelectric point was ascribed to a large difference in the formation between D-HA± · CD and L-HA± · CD.
Journal
-
- BUNSEKI KAGAKU
-
BUNSEKI KAGAKU 51 (10), 871-876, 2002
The Japan Society for Analytical Chemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679029056640
-
- NII Article ID
- 10009959094
-
- NII Book ID
- AN00222633
-
- COI
- 1:CAS:528:DC%2BD38XotFKntrg%3D
-
- NDL BIB ID
- 6315975
-
- ISSN
- 05251931
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed