ルミノール反応に基づく還元物質の化学発光

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書誌事項

タイトル別名
  • Chemiluminescence of reducing agents based on the luminol reaction.
  • ルミノール ハンノウ ニ モトズク カンゲン ブッシツ ノ カガク ハッコウ

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説明

The chemiluminescence reaction of luminol with reducing agents was studied by a flowinjection analysis. We found that reducing agents directly produce a chemiluminescence with luminol in the presence of hexacyanoferrate (III) and hexacyanoferrate (II) as a catalyst in an alkaline solution. To explain the chemiluminescence of luminol with reducing agents, ESR measurements were performed. ESR showed that superoxide anion radicals were generated from uric acid, dihydroxyacetone, hydroxylamine, cortisone and glucose in an alkaline solution by using 5, 5'-dimethyl-l-pyrroline N-oxide (DMPO) as a spintrap agent. The ascorbate radical was observed for ascorbic acid without DMPO. For phenacyl alcohol, a new radical without DMPO was observed. It is reported that the superoxide anion radical is a crucial intermediate in luminol chemiluminescence and that the chemiluminescence is generated in the presence of free radicals. Therefore, it was suggested that these radicals resulted from the reducing agents in the alkaline solution participate in luminol chemiluminescence. The detection limits (S/N=3) of uric acid, phenacyl alcohol, hydroxylamine, ascorbic acid, dihydroxyacetone, cortisone and glucose were 40, 60, 70, 200, 430, 440 and 75 ng, respectively.

収録刊行物

  • 分析化学

    分析化学 47 (9), 599-603, 1998

    公益社団法人 日本分析化学会

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