パルス紫外レーザー誘起キャピラリー振動法のポリマー溶液キャピラリー電気泳動法への適用による無修飾DNA断片の高感度検出(「特集」キャピラリー電気泳動法の新展開)

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タイトル別名
  • New Developments in Capillary Electrophoresis. Development and Improvement of Capillary Electrophoresis. Highly sensitive detection of nonderivatized DNA fragments by applying a pulsed UV laser-induced capillary vibration method to polymer solution capillary electrophoresis.
  • キャピラリー電気泳動法の新展開　キャピラリー電気泳動法・手法の開発・改良　パルス紫外レーザー誘起キャピラリー振動法のポリマー溶液キャピラリー電気泳動法への適用による無修飾ＤＮＡ断片の高感度検出
  • パルス シガイ レーザー ユウキ キャピラリー シンドウホウ ノ ポリマー ヨ

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Pulsed UV laser-induced stationary wave capillary vibration (CVL) detection was applied to polymer solution capillary electrophoresis (CE) for a highly sensitive detection of nonderivatized DNA fragments. A waveguide excimer laser (smaller-sized and easier for practical use than a continuous-wave (CW) UV laser) was used as a CVL excitation source. A methylcellulose solution was used as a separation medium. It was found that the fluidity of a methylcellulose solution resulted in a long duration (>one day), as in the case of a free solution, even though the nano-second pulse energy was nearly one-thousand times as large as that of intensity-modulated CW laser light. Nonderivatized DNA fragments (ca. 60 μM) were used as a sample; both CE separation and CVL detection were performed. No fatal damage to the separation medium was observed. The lower detection limit (S/N=2) of the absolute amount was calculated to be 7 attomole, which showed that the sensitivity using a polymer solution was not inferior to that using a free solution. These results showed the feasibility of polymer-solution CE/pulsed CVL to the practical analysis of biopolymers, such as sequencing and DNA diagnosis.

収録刊行物

  • 分析化学

    分析化学 46 (6), 415-420, 1997

    公益社団法人 日本分析化学会

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