マイクロマシニング技術を用いる石英及びガラス製電気泳動チップの作製とそれらの基本特性評価

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タイトル別名
  • Micro-fabrication and analytical performances of quartz and glass microchips for electrophoresis.
  • マイクロマシニング ギジュツ オ モチイル セキエイ オヨビ ガラスセイ デン

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抄録

Microchips for capillary electrophoresis have been fabricated in small size on quartz or glass substrates. Each microchip is 11 mm × 28 mm in size and 1.5 mm in thickness. It has a sample loading channel, a separation channel and four reservoirs at the ends of each channel. To fabricate a quartz chip, we applied a novel bonding method with a 1% (w/v) hydrofluoric acid solution. Using fabricated microchips, we evaluated several of their analytical features. We have shown that the microchips permit quantitative sample injection (several 10 pl or less) with an adequate potential control, high-speed analysis (30 s) due to the high heat-dissipation capability and effective separation (the number of theoretical plates is over 2500 at separation length of 17 mm).

収録刊行物

  • 分析化学

    分析化学 47 (6), 361-368, 1998

    公益社団法人 日本分析化学会

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