Determination of total chromium in human urine by graphite furnace atomic absorption spectrometry after coprecipitation treatment

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  • SHIMIZU Tokuo
    Institute of Environmental Chemistry, Faculty of Engineering, Utsunomiya University
  • HIYAMA Toshiaki
    Department of Industrial Chemistry, Faculty of Technology, Tokyo Metropolitan University
  • SHIJO Yoshio
  • SAKAI Kaoru
    Institute of Environmental Chemistry, Faculty of Engineering, Utsunomiya University

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Other Title
  • 共沈‐グラファイト炉原子吸光法による尿中の全クロムの定量
  • キョウチン グラファイトロ ゲンシ キュウコウホウ ニヨル ニョウチュウ ノ
  • 共沈-グラファイト炉原子吸光法による尿中の全クロムの定量

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Abstract

The ppb order of total chromium in human urine was determined by graphite furnace atomic absorption spectrometry after the following pretreatments. Water-soluble inorganic salts in human urine such as sodium chloride were removed by a coprecipitation method. Chromium (VI) which is not collected by the ferric hydroxide coprecipitation method was reduced to chromium(III) by L-ascorbic acid and was coprecipitated with ferric hydroxide at pH 10. After aging for 30 min, the precipitate was filtered with a 0.45μm millipore filter. Coprecipitated organic materials such as protein were digested with nitric acid-hydrogen peroxide for 90 min. Since the analytical line of chromium is 357.9 nm, correction of the background absorption by the coprecipitated inorganic salts was made by using a higher-brilliant thermal cathode type deuterium lamp instead of the conventional deuterium lamps even in the measurements in the visible region. The coefficient of variation was 0.97 % at 5 ppb chromium in synthetic urine (n=7) and 3.30 % in 24 h human urine (n=4). The ppb order of total chromium was determined for the urine of 8 people, and satisfactory results were obtained.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 29 (10), 680-685, 1980

    The Japan Society for Analytical Chemistry

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