High-performance liquid chromatography of amino acids with on-column fluorescence derivatization for a neonatal mass-screening test.

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  • オンカラム誘導体化高速液体クロマトグラフィーによる新生児マススクリーニング血液ろ紙中のアミノ酸の定量
  • オン カラム ユウドウタイカ コウソク エキタイ クロマトグラフィー ニヨル
  • オンカラム誘導体化高速液体クロマトグラフィーによる新生児マススクリーニング血液濾紙中のアミノ酸の定量

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Abstract

A determination method of amino acids in a dried blood disc for a neonatal mass-screening test was developed. The method consists of extraction with a 4mM sodium borate aqueous solution from a dried blood disc treated with methanol-acetone (1: 1) and an on-column fluorometric derivatization. High-performance liquid chromatography was performed isocratically using an acetonitrile-4 mM sodium borate aqueous solution (14.6: 85.4, v/v) containing 2 mM o-phthalaldehyde and 2 mM N-acetyl-L-cysteine as a mobile phase. Amino acids (i.e. Tyr, Val, Met, Ile, Orn, Phe, Leu, Lys) and methylhistamine as an internal standard were eluted within fifteen minuits with excellent reproducibility.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 46 (2), 89-94, 1997

    The Japan Society for Analytical Chemistry

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