Spectrophotometric determination of serum bilirubin using diazonium salt of 4-nitro-o-anisidine as color reagent.

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  • ４‐ニトロ‐ｏ‐アニシジンのジアゾニウム塩を発色試薬として用いる吸光光度法による血清ビリルビンの定量
  • 4 ニトロ o アニシジン ノ ジアゾニウムエン オ ハッショク シヤク ト
  • 4-ニトロ-ο-アニシジンのジアゾニウム塩を発色試薬として用いる吸光光度法による血清ビリルビンの定量

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Abstract

We present an assay for serum bilirubin using a new diazonium salt of 4-nitro-o-anisidine which reacts readily with intermediate formed in the diazo coupling reaction of bilirubin. The values of the present method correlated well with the values obtained by Michaëlsson method and enzymatic method. However, in the determination of total and direct bilirubin in serum, the values by the present method were significantly higher than those of the Michaëlsson method and the enzymatic method. Thus obtained laboratory data were 0.30.6 mg/dl for the direct bilirubin and 0.51.6 mg/dl for the total bilirubin.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 41 (3), 147-152, 1992

    The Japan Society for Analytical Chemistry

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