Studies on variation of tryptophan metabolites level in rabbit plasma with preparation procedures by high performance liquid chromatography.
-
- MORITA Ikue
- Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
-
- MASUJIMA Tsutomu
- Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
-
- YOSHIDA Hisanobu
- Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
-
- IMAI Hideo
- Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
書誌事項
- タイトル別名
-
- Studies on Variation of Tryptophan Meta
この論文をさがす
抄録
Reversed phase high performance liquid chromatography with direct injection of plasma samples was applied to evaluate the variation of plasma tryptophan (Trp) metabolites level with sample preparation procedures. Fresh heparinized rabbit plasma showed the peaks corresponding to serotonin (5-HT), 5-hydroxy indole-3-acetic acid (5-HIAA), indole-3-acetic acid (IAA), indole-3-lactic acid (ILA), indole-3-propionic acid (IPA), kynurenine (Kyn), kynurenic acid (KA) in addition to tryptophan (Trp). By standing plasma at 37°C, 5-HT trended to decrease about 5%/h, but other metabolites levels were constant for at least 24 h. By standing platelet rich plasma (PRP) for 6 h, 5-HT and 5-HIAA increased. In addition, 5-hydroxy tryptophol (5-OH TrpOL), which was not found as a common metabolite in fresh plasma, was detected. By standing whole blood for 6 h, almost all metabolites except IAA and IPA increased by several times. Serum levels of 5-HIAA, 5-OH TrpOL and especially 5-HT were higher than that of plasma. Consequently, the plasma samples should be prepared just after collecting blood for the analysis of Trp-metabolites level.
収録刊行物
-
- 分析化学
-
分析化学 33 (7), E235-E240, 1984
公益社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679029997696
-
- NII論文ID
- 110002908984
-
- NII書誌ID
- AN00222633
-
- COI
- 1:CAS:528:DyaL2cXkvVyjsLw%3D
-
- NDL書誌ID
- 2995779
-
- ISSN
- 05251931
- http://id.crossref.org/issn/05251931
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可