Determination of betaines in plants by low-pH capillary electrophoresis as their phenacyl esters.

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  • ZHANG Jinghua
    Department of Applied Biological Chemistry, The University of Tokyo
  • OKUBO Akira
    Department of Applied Biological Chemistry, The University of Tokyo
  • YAMAZAKI Sunao
    Department of Applied Biological Chemistry, The University of Tokyo

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Other Title
  • フェナシルエステル化／低ｐＨキャピラリー電気泳動法による植物中のベタインの分析
  • フェナシルエステルカ テイ pH キャピラリー デンキ エイドウホウ ニヨル

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Abstract

A simple and rapid determination method for plant osmolyte, betaines, was developed using capillary electrophoresis (CE). Betaines were first converted to phenacyl esters; the reaction mixture was then applied directly to CE.. A low pH of around 3 of the migration buffer resulted in a high separation, not only between betaine esters, but also between the reagent and products. This also gave another advantage of omitting a pretreatment procedure of the reaction mixture. Six species of betaine esters were well determined by either 214 or 262 nm. As for glycinebetaine, a good linearity was shown in concentration ranging from 0.050 to 5.0 mM. The repeatability (n=5) of the migration time and the peak area were 0.61 % and 4.13 % as RSD, respectively. Interferences by amino acid esters were negligible, except for lysine ester. This method was applied to plant samples collected from salt-affected soils of north China, and proved to be suitable for the determination of the major constituent, glycinebetaine.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 46 (4), 275-279, 1997

    The Japan Society for Analytical Chemistry

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