Determination of betaines in plants by low-pH capillary electrophoresis as their phenacyl esters.
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- ZHANG Jinghua
- Department of Applied Biological Chemistry, The University of Tokyo
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- OKUBO Akira
- Department of Applied Biological Chemistry, The University of Tokyo
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- YAMAZAKI Sunao
- Department of Applied Biological Chemistry, The University of Tokyo
Bibliographic Information
- Other Title
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- フェナシルエステル化/低pHキャピラリー電気泳動法による植物中のベタインの分析
- フェナシルエステルカ テイ pH キャピラリー デンキ エイドウホウ ニヨル
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Description
A simple and rapid determination method for plant osmolyte, betaines, was developed using capillary electrophoresis (CE). Betaines were first converted to phenacyl esters; the reaction mixture was then applied directly to CE.. A low pH of around 3 of the migration buffer resulted in a high separation, not only between betaine esters, but also between the reagent and products. This also gave another advantage of omitting a pretreatment procedure of the reaction mixture. Six species of betaine esters were well determined by either 214 or 262 nm. As for glycinebetaine, a good linearity was shown in concentration ranging from 0.050 to 5.0 mM. The repeatability (n=5) of the migration time and the peak area were 0.61 % and 4.13 % as RSD, respectively. Interferences by amino acid esters were negligible, except for lysine ester. This method was applied to plant samples collected from salt-affected soils of north China, and proved to be suitable for the determination of the major constituent, glycinebetaine.
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 46 (4), 275-279, 1997
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679030038144
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- NII Article ID
- 110002907117
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- NII Book ID
- AN00222633
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- COI
- 1:CAS:528:DyaK2sXisFShsb4%3D
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- NDL BIB ID
- 4184628
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- ISSN
- 05251931
- http://id.crossref.org/issn/05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed