1,1,3,3-テトラメトキシプロパンの加水分解とその生成物マロンジアルデヒドの吸光光度定量

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タイトル別名
  • Hydrolysis of 1,1,3,3-tetramethoxypropane and spectrophotometric determination of its product, malondialdehyde.
  • １，１，３，３‐テトラメトキシプロパンの加水分解とその生成物マロンジアルデヒドの吸光光度定量
  • 1 1 3 3 テトラメトキシプロパン ノ カスイ ブンカイ ト ソノ セイセ

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説明

The determination of malondialdehyde (MDA) which is an end product of the hydrolysis of 1, 1, 3, 3-tetramethoxypropane (TMP) was investigated spectrophotometrically. TMP in aqueous solution, adjusted pH to 1, was completely hydrolyzed, after 5h at 25°C. The rate constant of hydrolysis was 2.78×10-4s-1. The determination of MDA is carried out as follows: take 1ml of a sample solution, which contain less than 6.5×10-6M of MDA, add 12ml of a solution, which has 6.9×10-2M thiobarbituric acid dissolved in 5×10-2M sodium hydroxide; after adjusting pH to 1 with hydrochloric acid, dilute this solution with water to a 15ml; put the sample in boiling water for 15min and after cooling for 2h at room temperature, read the absorbance value. The calibration curve was prepared from MDA obtained by the hydrolysis of TMP. The linear relationship between the concentration of MDA and the absorbance was in the range of 0.5×10-6M to 6.5×10-6M. The molar extinction coefficient was 1.4×105l mol-1cm-1 at the wavelength of 532nm. This procedure can be applied to the determination of hydroxy radical.

収録刊行物

  • 分析化学

    分析化学 40 (3), 143-147, 1991

    公益社団法人 日本分析化学会

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