Separation of aromatic amines by capillary zone electrophoresis using untreated fused silica tubing.

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  • KOIZUMI Hitoshi
    Department of Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
  • SASAMOTO Kikuo
    Department of Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
  • SUZUKI Yoshihito
    Department of Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

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Other Title
  • 未修飾溶融シリカを用いるキャピラリーゾーン電気泳動による芳香族アミンの分離
  • ミ シュウショク ヨウユウ シリカ オ モチイル キャピラリー ゾーン デンキ

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Abstract

The optimum separation conditions for five aromatic amines by capillary zone electrophoresis was described. Citrate/phosphate buffer solution was used as the running buffer. The operational conditions were optimized for five aromatic amines as follows: capillary, 700 mm (effective length: 500 mm)× 50 μm i.d. fused silica capillary; buffer solution, 25×10-3 moll-1 citrate/phosphate buffer (pH 4.5); applied voltage, 10 kV (142 Vcm-1); capillary temperature, 40°C; detection wavelength, 205 nm. Five aromatic amines could be completely separated within 12 min. In general, better resolution by CZE is obtained with a higher applied voltage. In this experiment, the resolution of aromatic amines was increased when a lower voltage was applied. For this reason, it is concluded that incomplete replacement of sample bands was caused by both increased electroosmotic flow and suppressed dissociation. The repeatability (n=5) of the migration time and the peak area for aromatic amines was better than 0.6% and 9.0% as RSD, respectively.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 45 (8), 771-776, 1996

    The Japan Society for Analytical Chemistry

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