未修飾溶融シリカを用いるキャピラリーゾーン電気泳動による芳香族アミンの分離

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  • Separation of aromatic amines by capillary zone electrophoresis using untreated fused silica tubing.
  • ミ シュウショク ヨウユウ シリカ オ モチイル キャピラリー ゾーン デンキ

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The optimum separation conditions for five aromatic amines by capillary zone electrophoresis was described. Citrate/phosphate buffer solution was used as the running buffer. The operational conditions were optimized for five aromatic amines as follows: capillary, 700 mm (effective length: 500 mm)× 50 μm i.d. fused silica capillary; buffer solution, 25×10-3 moll-1 citrate/phosphate buffer (pH 4.5); applied voltage, 10 kV (142 Vcm-1); capillary temperature, 40°C; detection wavelength, 205 nm. Five aromatic amines could be completely separated within 12 min. In general, better resolution by CZE is obtained with a higher applied voltage. In this experiment, the resolution of aromatic amines was increased when a lower voltage was applied. For this reason, it is concluded that incomplete replacement of sample bands was caused by both increased electroosmotic flow and suppressed dissociation. The repeatability (n=5) of the migration time and the peak area for aromatic amines was better than 0.6% and 9.0% as RSD, respectively.

収録刊行物

  • 分析化学

    分析化学 45 (8), 771-776, 1996

    公益社団法人 日本分析化学会

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