Separation of aromatic amines by capillary zone electrophoresis using untreated fused silica tubing.
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- KOIZUMI Hitoshi
- Department of Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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- SASAMOTO Kikuo
- Department of Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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- SUZUKI Yoshihito
- Department of Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
Bibliographic Information
- Other Title
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- 未修飾溶融シリカを用いるキャピラリーゾーン電気泳動による芳香族アミンの分離
- ミ シュウショク ヨウユウ シリカ オ モチイル キャピラリー ゾーン デンキ
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Abstract
The optimum separation conditions for five aromatic amines by capillary zone electrophoresis was described. Citrate/phosphate buffer solution was used as the running buffer. The operational conditions were optimized for five aromatic amines as follows: capillary, 700 mm (effective length: 500 mm)× 50 μm i.d. fused silica capillary; buffer solution, 25×10-3 moll-1 citrate/phosphate buffer (pH 4.5); applied voltage, 10 kV (142 Vcm-1); capillary temperature, 40°C; detection wavelength, 205 nm. Five aromatic amines could be completely separated within 12 min. In general, better resolution by CZE is obtained with a higher applied voltage. In this experiment, the resolution of aromatic amines was increased when a lower voltage was applied. For this reason, it is concluded that incomplete replacement of sample bands was caused by both increased electroosmotic flow and suppressed dissociation. The repeatability (n=5) of the migration time and the peak area for aromatic amines was better than 0.6% and 9.0% as RSD, respectively.
Journal
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- BUNSEKI KAGAKU
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BUNSEKI KAGAKU 45 (8), 771-776, 1996
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679030145024
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- NII Article ID
- 110002907081
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- NII Book ID
- AN00222633
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- NDL BIB ID
- 4012302
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- ISSN
- 05251931
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed