高性能水系サイズ排除クロマトグラフィー用多孔性ガラスのカラム充てん法とその性能評価

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タイトル別名
  • Column packing procedure of porous glass packing materials for high performance aqueous size exclusion chromatography and evaluation of the columns.
  • コウセイノウ スイケイ サイズ ハイジョ クロマトグラフィーヨウ タコウセイ

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Diol bonded porous glass packing materials of average particle diameter 5 or 10μm and of pore sizes 170 Å and 500 Å were packed into a 7.2 mm i.d.×25 cm length column by a slurry packing procedure. Slurry solvents were the mixtures of methanol and ethylene glycol which had appropriate viscosity for packing materials. Packing solvent was n-heptane which was immissible with the slurry solvent. A 5-g portion of porous glass was mixed with the slurry solvent and packed into a column in 5 min under the constant flow by regulating the pressure of the packing solvent up to 500 kg/cm2 with a pneumatic pump. Viscosity of the slurry solvent should be controlled to suit particle sizes and pore diameters of the packing materials by changing the composition of methanol and ethylene glycol. Efficiency of the column thus obtained was 12000 theoretical plates per 25 cm by using ethylene glycol as a test sample and water as the mobile phase. Exclusion limit of the packing materials of 170 Å pore size was about 200000 as pullulan and that of 500 Å pore size was 1000000. These columns can be used for aqueous size exclusion chromatography.

収録刊行物

  • 分析化学

    分析化学 34 (8), 492-496, 1985

    公益社団法人 日本分析化学会

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