Down-Sizing in Analytical Chemistry. Development of a colorimetric assay system of trace metals and proteins in serum using microtiter plates and sensitive reagents.

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  • MAKINO Tetsuo
    Department of Clinical Chemistry, Kanagawa Prefectural College of Nursing and Medical Technology, 1-5-1, Nakao, Asahi-ku, Yokohama 241-0815

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Other Title
  • ダウンサイズ化する分析化学　　血清中微量金属及びタンパク質のマイクロプレート比色分析システムの開発
  • 血清中微量金属及びタンパク質のマイクロプレート比色分析システムの開発
  • ケッセイ チュウ ビリョウ キンゾク オヨビ タンパクシツ ノ マイクロ プレート ヒショク ブンセキ システム ノ カイハツ

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Abstract

A new colorimetric assay system using microtiter plates is presented for the determination of trace metals and proteins in serum samples. The use of polystyrene microwells with about 400μl capacity and sensitive reagents made it possible to reduce the sample size and required reagent. Rapid determination methods for iron, copper and zinc in 20∼50μl of serum using a water-soluble pyridylazo dye were developed. Drug-bound iron e.g. saccharated ferric oxide, and transferrin-bound iron in serum were simultaneously determined using a combined system of kinetic and end-point assay. A system for serum zinc with a cationic porphyrin was threetimes more sensitive than that with the pyridylazo dye. A latex agglutination immunoassay was utilized for the determination of α2-macroglobulin and albumin in serum. The proposed systems are sensitive, accurate, precise, simple, and relatively inexpensive.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 49 (12), 921-927, 2000

    The Japan Society for Analytical Chemistry

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