Reduction of misacylation to the amber suppressor tRNA during site-specific incorporation of unnatural amino acids

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Incorporations of unnatural amino acids in proteins are a useful technique in medical and industrial fields. For practical usages, misacylation caused by engineered aminoacyl-tRNA synthetases can be one of the issues lowering the purity of the generated proteins. Since acceptor stems of tRNAs are known to possess identity elements specific for each amino acid, the misacylation is probably caused by non-specificity of the acceptor stems in tRNAs. In this paper, we modified an acceptor stem of tRNATyr, and tested aminoacylation of over 100 modified tRNAs. Finally, we found the acceptor stem sequence for the tRNA with less misacylation for both tyrosine and tyrosine derivative unnatural amino acids.

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  • 生産研究

    生産研究 62 (3), 225-233, 2010

    東京大学生産技術研究所

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