書誌事項
- タイトル別名
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- A Description of a Structure Determination Procedure of a Gap Junction Channel at 3.5A Resolution.
- 3 5 オングストローム ブンカイノウ デ ノ ギャップ ケツゴウ チャネル ノ コウゾウ ケッテイ テジュン
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Gap junctions, which consist of arrays of intercellular channels, permit the exchange of ions and small molecules between adjacent cells. Here, we describe the structural determination of a gap junction channel composed of connexin 26 at 3.5 Å resolution. During each step of the purification process, the protein was examined using electron microscopy and/or dynamic light scattering. Dehydration of the crystals improved the resolution limits. Phase refinement using multi-crystal averaging in conjunction with non-crystallographic symmetry averaging resulted in an electron density map for model building. The amino-acid sequence of a protomer structure was assigned to the electron density map.
収録刊行物
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- 日本結晶学会誌
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日本結晶学会誌 51 (6), 327-333, 2009
日本結晶学会
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詳細情報 詳細情報について
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- CRID
- 1390282679062291200
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- NII論文ID
- 10026107628
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- NII書誌ID
- AN00188364
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- COI
- 1:CAS:528:DC%2BC3cXhsVKgsL4%3D
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- ISSN
- 18845576
- 03694585
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- NDL書誌ID
- 10552557
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可