Protein Crystallography using Synchrotron Radiation. An Application of Multiwavelength Anomalous Diffraction Method.

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Other Title
  • 放射光利用によるタンパク質の結晶構造解析　多波長異常分散法の利用
  • ホウシャコウ リヨウ ニ ヨル タンパクシツ ノ ケッショウ コウゾウ カイセ
  • —An Application of Multiwavelength Anomalous Diffraction Method—
  • ―多波長異常分散法の利用―

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Synchrotron radiation (SR) is an extremely useful X-ray source for protein crystallography. It is high brilliance, high intensity and small divergence white X-ray source, which enables us to collect high resolution diffraction data of biological macromolecular crystals with large cell dimensions. Furthermore, wavelength tunability of SR is essential for the phase determination by multiwavelength anomalous diffraction (MAD) method, which has a possibility of the direct phase determination of metal proteins, selenomethionyl proteins and proteins with single isomorphous derivative of poor isomorphism.<BR>MAD method is applied for the structure determination of cytochrome c-553 from Desulfovibrio vulgaris Miyazaki F strain.

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