Structural Studies on Membrane Proteins in Biological Macromolecular Assemblies in Japan

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  • TSUKIHARA Tomitake
    Institute Protein Research, Osaka University Department of Life Science, University of Hyogo

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  • 構造生物学研究の新展開　　１．タンパク質構造研究に対する結晶学の役割　　わが国における膜タンパク質と超分子の構造研究
  • わが国における膜タンパク質と超分子の構造研究
  • ワガクニ ニ オケル マク タンパクシツ ト チョウブンシ ノ コウゾウ ケンキュウ

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Abstract

Structural studies on membrane proteins have been performed at atomic level by both three-dimensional X-ray crystallography and two-dimensional electron crystallography in Japan as in Europe and Unites States. More than 13 membrane protein structures were elucidate by X-ray method in our country, and seven membrane protein structures were determined by cryo-electron microscopic method developed by Fujiyoshi of Kyoto University. Extensive crystallographic studies on calcium pump and cytochrome c oxidase elucidated their functional mechanisms at atomic level.<br>Structure and switching mechanism of a flagellum were studied by X-ray and electron microscopic methods. Vault structure exhibiting D39 symmetry was determined by X-ray method.

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