Supporting Strategies of Protein Crystallization by Dynamic Light Scattering.
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- KATO Hiroaki
- Institute for Chemicl Research, Kyoto University
Bibliographic Information
- Other Title
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- 動的光散乱法を用いたタンパク質の結晶化の支援法
- ドウテキ ヒカリ サンランホウ オ モチイタ タンパクシツ ノ ケッショウカ
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Abstract
The purpose of this technical guide is to describe how dynamic light scattering (DLS) is employed to screen crystallization candidates. Many findings suggest that DLS is useful to evaluate crystallizability of macromolecules before any crystallization attempt. The description is started with an overview of the relation between the crystallizability of macromolecular solution and its monodispersity provided by the DLS measurement. A practical guide for the DLS experiment is also described briefly in the case of using a compact commercial product, Dyna-Pro form Protein Solutions, Inc. Finally, the case study we have done in some protein samples is presented.
Journal
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- Nihon Kessho Gakkaishi
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Nihon Kessho Gakkaishi 39 (4), 315-319, 1997
The Crystallographic Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679064044288
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- NII Article ID
- 10002589871
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- NII Book ID
- AN00188364
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- ISSN
- 18845576
- 03694585
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- NDL BIB ID
- 4292562
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed
