Growth of Protein Crystals in Hydrogels with High Strength
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- SUGIYAMA Shigeru
- Graduate School of Science, Osaka University
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- HIROSE Mika
- Graduate School of Science, Osaka University
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- SAZAKI Gen
- The Institute of Low Temperature Science, Hokkaido University
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- ADACHI Hiroaki
- SOSHO, Inc.
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- TAKANO Kazufumi
- Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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- MURAKAMI Satoshi
- Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
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- MATSUMURA Hiroyoshi
- Graduate School of Engineering, Osaka University
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- MARUYAMA Mihoko
- Graduate School of Engineering, Osaka University
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- INOUE Tsuyoshi
- Graduate School of Engineering, Osaka University
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- MORI Yusuke
- Graduate School of Engineering, Osaka University
Bibliographic Information
- Other Title
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- 固相ハイドロゲル中でのタンパク質結晶化
- ジッケンシツ コソウ ハイドロゲル チュウ デ ノ タンパクシツ ケッショウカ
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Abstract
High-throughput X-ray protein crystallography offers unprecedented facilitation to structure based drug discovery. The popular way to prepare crystals of target protein in complex with a candidate compound is the soaking method. However, the method often causes damage due to osmotic pressure to crystals, especially when a compound is dissolved in an organic solvent or solution with high ionic strength. These solutions damage protein crystals by osmotic shock. To overcome this difficulty, we have developed a novel technique to grow protein crystals in the concentrated hydrogel. Here, we demonstrate that protein crystals grown in hydrogel are stable to soaking and suffer less damage.
Journal
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- Nihon Kessho Gakkaishi
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Nihon Kessho Gakkaishi 54 (5), 300-303, 2012
The Crystallographic Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679065341824
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- NII Article ID
- 10031129966
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- NII Book ID
- AN00188364
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- ISSN
- 18845576
- 03694585
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- NDL BIB ID
- 024085233
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed