Serial Femtosecond Crystallography at SACLA: Current Situation and Future Prospects
-
- NANGO Eriko
- RIKEN SPring-8 Center
-
- NAKANE Takanori
- Graduate School of Science, The University of Tokyo
-
- IWATA So
- RIKEN SPring-8 Center Graduate School of Medicine, Kyoto University
Bibliographic Information
- Other Title
-
- SACLAにおけるシリアルフェムト秒結晶構造解析の現状と展望
- SACLA ニ オケル シリアルフェムトビョウ ケッショウ コウゾウ カイセキ ノ ゲンジョウ ト テンボウ
Search this article
Abstract
<p>Intense, femtosecond X-ray pulses from X-ray free electron laser (XFEL) have enabled acquisition of diffraction patterns from protein microcrystals before the onset of radiation damage. Although a single-shot exposure of XFEL destroys a crystal, diffraction by XFEL terminates before movements of atoms become significant. Thus, XFEL allows determination of damage free structures at room temperature. In serial femtosecond crystallography, fresh microcrystals are continuously delivered by an injector to the XFEL interaction zone. When combined with an excitation laser in a pump-probe setting, this technique is of great utility for time-resolved experiments in measuring ultra-fast reactions in proteins. We will describe current situation of SFX experiments at SACLA and future prospects.</p>
Journal
-
- Nihon Kessho Gakkaishi
-
Nihon Kessho Gakkaishi 59 (1), 12-17, 2017
The Crystallographic Society of Japan
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282679065413248
-
- NII Article ID
- 130005437788
-
- NII Book ID
- AN00188364
-
- ISSN
- 18845576
- 03694585
-
- NDL BIB ID
- 028037209
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed