書誌事項
- タイトル別名
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- Structure and Function of a Novel Chitinase Belonging to GH Family 23
- GH ファミリー 23 ニ ゾクスル シンキ キチナーゼ ノ コウゾウ ト キノウ
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抄録
Chitinase C from a moderate thermophilic strain Ralstonia sp. A-471 (Ra-ChiC) is a novel chitinase which has a catalytic domain sequence similar to goose type lysozymes and, unlike other chitinases, Ra-ChiC belongs to glycoside hydrolase family 23. We have determined the crystal structures of Ra-ChiC catalytic domain and its inactive mutant with or without chitin oligosaccharides. These structures indicated that Ra-ChiC has a unique substrate-binding site including a tunnel-shaped cavity. In addition, our mutation analysis showed that not only a highly conserved Glu141 but also Asp226 located at the roof of the tunnel have quite important roles in catalysis.
収録刊行物
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- 日本結晶学会誌
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日本結晶学会誌 56 (3), 201-206, 2014
日本結晶学会
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詳細情報 詳細情報について
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- CRID
- 1390282679065547136
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- NII論文ID
- 130004552901
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- NII書誌ID
- AN00188364
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- ISSN
- 18845576
- 03694585
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- NDL書誌ID
- 025636332
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
