ヒトMAPキナーゼJNK1の立体構造・機能・安定性に対する遊離システイン残基の置換効果

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  • Seven Cysteine-Deficient Mutants Depict Interplay between Thermal and Chemical Stabilities of Individual Cysteine Residues in MAP Kinase JNK1
  • ヒト MAP キナーゼ JNK1 ノ リッタイ コウゾウ ・ キノウ ・ アンテイセイ ニ タイスル ユウリ システインザンキ ノ チカン コウカ

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To characterize the role of cysteine residues on the structure, function and stability of JNK1, we prepared and evaluated the wild-type JNK1 and seven cysteine-deficient JNK1 proteins. The solvent exposed cysteine residues did not influence biological function and mutating these residues raised the thermal stability because of newly formed hydrogen bonds and of higher hydration as speculated by the mutant structures. The surface cysteine involved in the molecular-surface hydrophobic pocket did not affect biological function; although a moderate thermal destabilization was observed. Cysteines in the loosely-assembled hydrophobic environment moderately contributed to thermal stability and the mutations of these cysteines had negligible effect on enzyme activity. The other cysteines are involved in the tightly-filled hydrophobic core and mutation of these residues conferred the adverse effects on the thermal stability and enzyme activity.

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