書誌事項
- タイトル別名
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- Visualization of Nucleotidyl Transfer Reaction by Human DNA Polymerase η Using Time Resolved Protein Crystallography
- ジブンカツ タンパクシツ ケッショウガク ニ ヨル DNA ポリメラーゼe ノ ヌクレオチド テンイ ハンノウ ノ カシカ
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抄録
We visualized the course of nucleotidyl transfer reaction by human DNA polymerase η (Pol η) using time resolved protein crystallography. The reaction was initiated by exposing Pol η-DNA-dATP crystals to 1 mM Mg2+ at pH 7.0 and stopped at various time points by freezing. The substrates and two Mg2+ ions are aligned for reaction within 40 s. Transient electron densities indicate that deprotonation and an accompanying C2'-endo to C3'-endo conversion of the nucleophile 3'-OH are rate limiting. A third Mg2+ ion, which arrives with the new bond and stabilizes the intermediate state, may be an unappreciated feature of the two-metal-ion mechanism.
収録刊行物
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- 日本結晶学会誌
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日本結晶学会誌 55 (1), 42-46, 2013
日本結晶学会
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詳細情報 詳細情報について
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- CRID
- 1390282679067175680
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- NII論文ID
- 10031156005
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- NII書誌ID
- AN00188364
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- COI
- 1:CAS:528:DC%2BC3sXnvVelt7o%3D
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- ISSN
- 18845576
- 03694585
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- NDL書誌ID
- 024334927
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可
