Whole-Body β-Oxidation of Docosahexaenoic Acid Is Decreased under n-3 Polyunsaturated Fatty Acid Insufficiency as Assessed by a 13CO2 Breath Test Using [U-13C]Fatty Acids
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- WAKI Hatsue
- Neuronal Function Research Group, Tokyo Metropolitan Institute of Gerontology
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- WATANABE Kazuo
- Microbial Engineering Group, Sagami Chemical Research Center
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- ISHII Hitoshi
- Arcosystem INC.
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- TANAKA Yasukazu
- Neuronal Function Research Group, Tokyo Metropolitan Institute of Gerontology
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- SHUMIYA Seigo
- Laboratory Animal Facilities, Tokyo Metropolitan Institute of Gerontology
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- ANDO Susumu
- Neuronal Function Research Group, Tokyo Metropolitan Institute of Gerontology
書誌事項
- タイトル別名
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- Whole-Body .BETA.-Oxidation of Docosahexaenoic Acid Is Decreased under n-3 Polyunsaturated Fatty Acid Insufficiency as Assessed by a 13CO2 Breath Test Using [U-13C]Fatty Acids
- Whole Body ベータ Oxidation of Docosahexaenoic Acid Is Decreased under n 3 Polyunsaturated Fatty Acid Insufficiency as Assessed by a 13CO2 Breath Test Using U 13C Fatty Acids
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We applied a continuous 13CO2 breath test to examination of the effects of n-3 polyunsaturated fatty acid (PUFA) insufficiency on fatty acid β-oxidation. Fischer rats were divided into two groups, and were reared on two different regimens of diet for multiple successive generations. One diet was adequate with respect to n-3 PUFA and the other was insufficient in n-3 PUFA. Rats (the F18 generation) were given [U-13C]docosahexaenoic acid or [U-13C]palmitic acid by intravenous injection, and expired 13CO2 was continuously measured using a mass spectrometer. Fatty acid oxidation was evaluated by two parameters, the half-life of 13CO2 in the breath and cumulative 13CO2 recovery from the labeled fatty acids. When [U-13C] docosahexaenoic acid was administered, cumulative 13CO2 recovery was 40% lower in the n-3 PUFA-insufficient diet group than in the n-3 PUFA-adequate diet group. The half-life appeared to be the same for the two diet groups. In the case of [U-13C]palmitic acid, neither the half-life nor cumulative 13CO2 recovery significantly differed between the two diet groups. These results suggest that overall β-oxidation of DHA in a whole-body system is decreased under n-3 PUFA insufficiency. Our finding shows that the 13CO2 breath test can be used to assess n-3 PUFA insufficiency. The test provides a useful and invasive method for evaluation of fatty acid β-oxidation.
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 53 (3), 135-142, 2004
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679067551488
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- NII論文ID
- 130000055741
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- NII書誌ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 6851079
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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