糸状菌<I>Mortierella alpina</I>の必須ミネラルに関する研究

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タイトル別名
  • Minerals Essential for Growth of the Filamentous Fungus, <I>Mortierella alpina</I>
  • シジョウキン Mortierella alpina ノ ヒッス ミネラル ニ カンスル ケンキュウ

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説明

Potato protein and dextrose are ideal media for cultivation of Mortierella alpina, that produces arachidonic acid. Corn peptide-dextrose medium is not suitable for growth of the fungus although amino acid compositions of the two are similar. Thus, to corn peptide- dextrose medium was added metallic salt containing quasi-major element, sodium, calcium, magnesium, phosphor, or potassium in amounts from 0.050.30% and used for liquid cultivation at 20°C in a 500-mL shake flask for 14 days. Cell growth was greatly enhanced but the lipid accumulation ratio of methyl esters (g) /dry cell weight (g) reached only 70% that of lipid in cells grown in potato-dextrose medium. Iron, copper, zinc, or manganese was then added at 2×10-51×10-3% to corn peptide-dextrose medium on top of phosphor and potassium for cultivation. Iron and manganese increased the lipid accumulation ratio to essentially the same extent as above with nitrogen content in the medium the same. Phosphor and potassium may be concluded essential for the growth of M. alpina and iron and manganese to have significant roles in lipid synthesis.

収録刊行物

  • 日本油化学会誌

    日本油化学会誌 49 (5), 487-493,515, 2000

    公益社団法人 日本油化学会

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