Stereospecific Analysis of Triacyl-<I>sn</I>-glycerols by HPLC

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  • ANDO Yasuhiro
    Department of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University

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  • HPLCによるトリアシルグリセロールの立体特異分析
  • HPLC ニ ヨル トリアシルグリセロール ノ リッタイ トクイ ブンセキ

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Abstract

High-performance liquid chromatography (HPLC) for stereospecific analysis of triacyl-sn-glycerols (TG) does not have the drawbacks attending enzymatic methods and involves the separation of isomeric and enantiomeric partial acylglycerols, i.e., monoacylglycerols (MG) and diacylglycerols (DG), farmed from TG. In this method, the Grignard hydrolysis of TG to partial acylglycerols and their isolation are carried out followed by the preparation of enantiomeric or diastereomeric urethane derivatives, their resolution by chiral- or achiral-phase HPLC and fatty acid analysis as methyl esters. The accuracy of this method depends on acyl group migration during analysis, differential loss in conversion, resolution of enantiomers and calculation for acyl compositions in sn-1, sn-2, and sn-3 positions. Soybean, pine seed, yew seed, and saucy oil TG have been analyzed.

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