Novel Pre-fractionation Method of Trans Fatty Acids by Gas Chromatography with Silver-Ion Cartridge Column
-
- Goto Hirofumi
- Japan Food Research Laboratories, Nagoya Branch Mimasaka University
-
- Shionoya Noriko
- Japan Food Research Laboratories, Nagoya Branch
-
- Sugie Megumi
- Japan Food Research Laboratories, Nagoya Branch
-
- Tominaga Makoto
- Japan Food Research Laboratories, Nagoya Branch
-
- Shimelis Olga
- Supelco
-
- Taniguchi Makoto
- Mimasaka University
-
- Igarashi Tomoji
- Japan Food Research Laboratories, Nagoya Branch
-
- Hirata Yoshiaki
- Japan Food Research Laboratories, Nagoya Branch
この論文をさがす
抄録
We developed a novel pre-separation method of trans fatty acids (TFAs) using a silver-ion cartridge column and GC. As a preliminary study, a mixture of fatty acid methyl esters consisting of saturated, cis-unsaturated, and trans-unsaturated fatty acids was dissolved in dichloromethane and loaded onto a Bond Elut SCX ion-exchange cartridge column that was converted to the silver-ion form. The column was then eluted with dichloromethane to obtain the saturated fatty acids, dichloromethane/ethyl acetate (90/10) for the trans mono-ene, dichloromethane/ethyl acetate (65/35) for the cis mono-ene, dichloromethane/acetone (60/40) for the trans di-ene, and acetone/acetonitrile (80/20) for the others. Satisfactory separation of the cis/trans isomers was confirmed by GC analysis. To generalize this technique, the elution conditions of the ready-to-use Discovery Ag-ION SPE cartridge column were also optimized. Both cartridge columns had good separation, recovery, and repeatability. Peer laboratory verification was carried out between two laboratories using different production lots of the ready-to-use cartridge column, and the robustness of the product and reproducibility of the method were found to be satisfactory. This technique is therefore a powerful tool not only for routine analyses of TFAs in oils, fats, and foods but also for detailed analyses of TFAs in various research fields.
収録刊行物
-
- Journal of Oleo Science
-
Journal of Oleo Science 61 (2), 49-56, 2012
公益社団法人 日本油化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679068333696
-
- NII論文ID
- 130001882207
-
- NII書誌ID
- AA11503337
-
- COI
- 1:STN:280:DC%2BC387ot1Ogug%3D%3D
-
- ISSN
- 13473352
- 13458957
-
- NDL書誌ID
- 023388125
-
- PubMed
- 22277887
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可