Glycolipid Biosurfactants, Mannosylerythritol Lipids, Show Antioxidant and Protective Effects against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress in Cultured Human Skin Fibroblasts

  • Takahashi Makoto
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central Present address: Faculty of Agriculture, University of the Ryukyus
  • Morita Tomotake
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central
  • Fukuoka Tokuma
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central
  • Imura Tomohiro
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central
  • Kitamoto Dai
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central

Bibliographic Information

Other Title
  • Glycolipid Biosurfactants, Mannosylerythritol Lipids, Show Antioxidant and Protective Effects against H₂O₂-Induced Oxidative Stress in Cultured Human Skin Fibroblasts

Search this article

Description

Mannosylerythritol lipids (MELs) are biosurfactants known for their versatile interfacial and biochemical properties. To broaden their application in cosmetics, we investigated the antioxidant properties of different MEL derivatives (MEL-A, -B, and -C) by using a 1,1-diphenyl-2-picryl hydrazine (DPPH) free-radical- and superoxide anion-scavenging assay. All MEL derivatives tested showed antioxidant activity in vitro, but at lower levels than those of arbutin. Of the MELs, MEL-C, which is produced from soybean oil by Pseudozyma hubeiensis, showed the highest rates of DPPH radical scavenging (50.3% at 10 mg/mL) and superoxide anion scavenging (>50% at 1 mg/mL). The antioxidant property of MEL-C was further examined using cultured human skin fibroblasts (NB1RGB cells) under H2O2-induced oxidative stress. Surprisingly, MEL-C had a higher protective activity against oxidative stress than arbutin did: 10 μg/mL of MEL-C and arbutin had protective activities of 30.3% and 13%, respectively. Expression of an oxidative stress marker, cyclooxygenase-2, in these cells was repressed by treatment with MEL-C as well as by arbutin. MEL-C was thus confirmed to have antioxidant and protective effects in cells, and we suggest that MELs have potential as anti-aging skin care ingredients.

Journal

Citations (4)*help

See more

References(21)*help

See more

Details 詳細情報について

Report a problem

Back to top