Surface Modification of Reconstituted Hemoglobins Containing SOD-Active Metalloporphyrins

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  • YUASA Makoto
    Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science Institute of Colloid and Interface Science, Tokyo University of Science
  • OYAIZU Kenichi
    Institute of Colloid and Interface Science, Tokyo University of Science
  • YAMAGUCHI Aritomo
    Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science
  • HAYASHI Takamitsu
    Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science
  • MIDORIKAWA Uichi
    Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science

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  • ＳＯＤ活性金属ポルフィリンを含む再構成ヘモグロビンの表面改質（原標題は英語）

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Reconstituted hemoglobins modified with polyethylene glycol (PEG) containing iron(III) and manganese(III) complexes of meso-tetra(N-methylpridinium-x-yl)porphyrin ([MTxMePyP]-Hb-PEG, M = Fe and Mn, x = 2 and 4) were found to serve as excellent superoxide dismutase (SOD) mimics under physiological conditions. IC50 was 2.3 μ M and the pseudo-first order rate constant was 2.2 × 106 M-1s-1 for reaction of MnT2MePyP-Hb-PEG with a superoxide anion radical as detemined by conventional cytochrome c assay and stopped flow analysis, respectively. Stability toward H2O2 by surface modification of reconstituted hemoglobins with PEG chains helped prevent aggregation with other proteins such as albumin.<br>

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