Chimyl Alcohol Suppresses PGE₂ Synthesis by Human Epidermal Keratinocytes through the Activation of PPAR-γ
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- Yokota Mariko
- NIKKOL GROUP Cosmos Technical Center Co., Ltd. Laboratory of Dermatological Physiology, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University
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- Yahagi Shoichi
- NIKKOL GROUP Cosmos Technical Center Co., Ltd.
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- Tokudome Yoshihiro
- Laboratory of Dermatological Physiology, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University
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- Masaki Hitoshi
- School of Bioscience and Biotechnology, Tokyo University of Technology
書誌事項
- タイトル別名
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- Chimyl Alcohol Suppresses PGE<sub>2</sub> Synthesis by Human Epidermal Keratinocytes through the Activation of PPAR-γ
- Chimyl Alcohol Suppresses PGE2 Synthesis by Human Epidermal Keratinocytes through the Activation of PPAR-gamma
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説明
<p>Alkyl glyceryl ethers (AKGs) are widely used as emulsion stabilizers, and their anti-inflammatory effects are well known. Daily exposure to environmental stresses, such as chemicals, low humidity and ultraviolet light (UV), can initiate and promote the development of various skin problems. Among those stresses, it has been established that UV induces skin pigmentation and accelerates premature skin aging due to the inflammation that results. Here, we investigated whether chimyl alcohol (CA), which is an AKG, suppresses the inflammatory process. The suppression of cell damage and the reduction of intracellular levels of reactive oxygen species (ROS) in normal human epidermal keratinocytes (NHEKs) after UVB exposure was evaluated using the Neutral red (NR) and the 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) assays, respectively. Moreover, the expression levels of mRNAs and proteins related to inflammation were evaluated by Real-time RT-PCR and ELISA assays, respectively. CA suppressed prostaglandin E2 (PGE2) production in UVB-exposed NHEKs according to the down-regulated expression level of cyclooxygenase-2 (COX-2) mRNA. Furthermore, CA up-regulated the mRNA expression levels of peroxisome proliferator-activated receptor (PPAR)-γ, nuclear factor E2-related factor 2 (Nrf2) and γ-glutamyl cysteine synthase (γ-GCS) in NHEKs. Finally, we examined the effects of CA on siPPAR-γ transfected NHEKs. siPPAR-γ transfection of NHEKs abolished the mRNA expression levels of Nrf2 and UVB-stimulated PGE2 secretion that were regulated by CA. Hence, CA suppresses the UVB-induced COX-2 mRNA expression and PGE2 production through PPAR-γ as an agonist. We conclude that CA provides useful protection and/or alleviation against UV damage.</p>
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 67 (4), 455-462, 2018
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679069233280
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- NII論文ID
- 130006602054
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- NII書誌ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 028898138
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- PubMed
- 29526871
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
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- 使用不可