Quantitative Analysis of the Accumulation of Marine-derived Tocopherol in the Tissue of Mice Fed with Salmon Roe Oil Using HPLC-fluorescence

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  • Beppu Fumiaki
    Department of Food Science and Technology, Tokyo University of Marine Science and Technology
  • Kondo Hitomi
    Department of Food Science and Technology, Tokyo University of Marine Science and Technology
  • Kasatani Satoshi
    Laboratory Section Division III, MITSUBISHI-CHEMICAL FOODS CORPORATION
  • Aoki Yoshinori
    Health Food Ingredient Section, Sales and Marketing Department Division III, MITSUBISHI-CHEMICAL FOODS CORPORATION
  • Gotoh Naohiro
    Department of Food Science and Technology, Tokyo University of Marine Science and Technology

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<p>In this study, we measured the quantity of marine-derived tocopherol (MDT), a monounsaturated vitamin E (VE), stored in the body tissue of mice fed with a diet containing a VE-rich fraction extracted from salmon roe. We first prepared the calibration curves for the MDT concentration using an HPLC-fluorescence system. Ranging from 0.016 to 50 μg/mL, the slope was expressed as first-order equations, with R2 values = 0.99. The mice were fed with an AIN-93 based diet containing MDT in doses of 21.4 mg/kg for 4 weeks, and the storage in the heart, lung, liver, stomach, small intestine, large intestine, kidney, pancreas, spleen, testis, skeletal muscle, visceral white adipose tissue (WAT), subcutaneous WAT and brain was quantified. MDT was widely distributed in tissues throughout the whole body, with higher accumulations observed in the adipose tissue, liver and kidney. These results demonstrate means to estimating the MDT concentration in natural products and in the bodies of animals and contribute to the understanding of the physiological functions of MDT in relation to human health.</p>

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