Synthesis of [3H]-Labeled Bioactive Lipid A Analogs and Their Use for Detection of Lipid A-Binding Proteins on Murine Macrophages.

DOI PDF Web Site 被引用文献5件 参考文献64件
  • Fukase Koichi
    Department of Chemistry, Graduate School of Science, Osaka University
  • Kirikae Teruo
    Department of Infectious Diseases and Tropical Medicine, Research Institute, International Center
  • Kirikae Fumiko
    Department of Infectious Diseases and Tropical Medicine, Research Institute, International Center
  • Liu Wen-Chi
    Department of Chemistry, Graduate School of Science, Osaka University
  • Oikawa Masato
    Department of Chemistry, Graduate School of Science, Osaka University
  • Suda Yasuo
    Department of Chemistry, Graduate School of Science, Osaka University
  • Kurosawa Motohiro
    Department of Chemistry, Graduate School of Science, Osaka University
  • Fukase Yoshiyuki
    Department of Chemistry, Graduate School of Science, Osaka University
  • Yoshizaki Hiroaki
    Department of Chemistry, Graduate School of Science, Osaka University
  • Kusumoto Shoichi
    Department of Chemistry, Graduate School of Science, Osaka University

書誌事項

公開日
2001
DOI
  • 10.1246/bcsj.74.2189
公開者
公益社団法人 日本化学会

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説明

Both endotoxic and antagonistic [3H]-labeled 2-(phosphonooxy)ethyl (PE) analogs of lipid A were synthesized with high purity and high specific radioactivity. Lipid A-binding proteins were detected by using the endotoxic analog of hexaacyl Escherichia coli-type designated [3H] PE-506. The plasma membrane fractions from peritoneal macrophages derived from LPS-responder C3H/HeN mice and LPS-hyporesponder C3H/HeJ mice were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The membranes were then incubated with the [3H] PE-506. Several [3H] PE-506 binding proteins were detected in both C3H/HeN and C3H/HeJ macrophages. Unlabeled hexaacyl lipid A inhibited the interaction between [3H] PE-506 and these proteins. The result suggests that there exist multiple binding sites for lipid A on macrophages. LPS-induced change in the profile of the cell surface lipid A binding proteins was observed in C3H/HeN macrophages, but not in C3H/HeJ macrophages, by preincubation of macrophages with LPS.

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