Enzymatic Microdetermination of Nitrate

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1) On the principle of the colorimetry of nitrite produced by enzymatic reduction, a method of microdetermination of nitrate was established.<BR>2) This method can be applied to 10–100μM/1. nitrate solution. 2 ml. of the solution (1.2–12μg. NO−3 or 0.28–2.8μg. nitrate-N) is sufficient for an analysis.<BR>3) This method is successfully applied to the estimation of nitrate in human urine, horse serum, spinach leaf extract, sea water and rain water.

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