Direct Measurement and Quantification of Drugs and Their Metabolites in Urine by MALDI-MS Using Zeolite

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  • Kitaoka Chihiro
    Criminal Investigation Laboratory, Metropolitan Police Department
  • Asano Takashi
    Criminal Investigation Laboratory, Metropolitan Police Department
  • Fujino Tatsuya
    Department of Applied Chemistry, Graduate School of Science and Engineering, Toyo University

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<p>A complex of α-cyano-4-hydroxycinnamic acid (CHCA) and zeolite was used as the matrix for the analysis of drugs and their metabolites in urine by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). It was found that acetaminophen (AAP) and its metabolites could be detected in urine without any pretreatment or separation process. It was also found that the hydrolysis of one of the metabolites, glucuronide, was promoted when a mixture of urine and the developed matrix was heated at 343 K for 15 minutes. Because of the homogeneous distribution of CHCA on the zeolite surface, high reproducibility of the analyte peak intensity was achieved. By using isotope-labeled acetaminophen (D4-AAP) as the internal standard, quantitative analysis of AAP in urine from a donor who took 300 mg of AAP four hours before was performed, and 5.29 ± 0.19 mg of AAP was detected in 1.00 g of urine.</p>

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