Identification of promoter sequences using archaeal genomic sequences 2. Analysis of a meso-thermophile, Thermoplasma volcanium

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  • AMANO Naoki
    National Institute of Advanced Industrial Science and Technology (AIST)
  • TSUJI Kuniaki
    National Institute of Advanced Industrial Science and Technology (AIST)
  • EBIHARA Sonomi
    National Institute of Advanced Industrial Science and Technology (AIST)
  • SUZUKI Masashi
    National Institute of Advanced Industrial Science and Technology (AIST)

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  • 2. Analysis of a meso-thermophile, <i>Thermoplasma volcanium</i>

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Following the direction of our previous publication (Amano, N., Tsuji, K., and Suzuki, M. (2003) Proc. Japan Acad. 79B, 131-136), promoter and gene-coding regions, and types of transcription units (i.e. operons and independently regulated genes) were theoretically identified for Thermoplasma volcanium. Possibly reflecting the optimum growth temperature lower than that of Pyrococcus sp. OT3, signals for transcription and translation were found to be weaker, and the degree of differentiating gene-coding regions was lesser. Nevertheless, with some modification of the strategy, genes were identified with a similarly high precision. Some notable characteristics of the identified promoter sequences are discussed.<BR><BR>(Communicated by Masanori OTSUKA, M. J. A., June 10, 2003)

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