Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection

DOI Web Site Web Site 参考文献16件
  • AOKI Takaaki
    Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University
  • HIRANO Minako
    Laboratory for Nanobiology, Graduate School of Frontier Biosciences, Osaka University
  • TAKEUCHI Yuko
    Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University
  • KOBAYASHI Toshihide
    Lipid Biology Laboratory, RIKEN
  • YANAGIDA Toshio
    Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University Laboratory for Nanobiology, Graduate School of Frontier Biosciences, Osaka University
  • IDE Toru
    Network Center for Molecular and System Life Science, Graduate School of Frontier Biosciences, Osaka University Molecular-Informational Life Science Research Group, RIKEN

この論文をさがす

抄録

Single channel currents of lysenin were measured using artificial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl2, and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio PNa/PCl was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were influenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration-dependent with applied DNA. As the current fluctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current fluctuations.<BR><BR>(Communicated by Fumio OOSAWA, M.J.A.)

収録刊行物

参考文献 (16)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ