Production and chromosomal features of triploid hybrids between Rhodeus ocellatus ocellatus and Acheilognathus limbatus (Cyprinidae, Pisces).

OJIMA Yoshio, TAKAI Akinori, YAMAMOTO Kazuhiro

doi:10.2183/pjab.64.225

Production and chromosomal features of triploid hybrids between Rhodeus ocellatus ocellatus and Acheilognathus limbatus (Cyprinidae, Pisces).

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OJIMA Yoshio

Department of Biology, Faculty of Science, Kwansei Gakuin University
TAKAI Akinori

Department of Biology, Faculty of Science, Kwansei Gakuin University
YAMAMOTO Kazuhiro

Department of Biology, Faculty of Science, Kwansei Gakuin University

書誌事項

タイトル別名

Cyprinidae, Pisces

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説明

Triploid hybrids between Rhodeus ocellatus ocellatus (female) and Acheilognathus limbatus (male) were artificially induced by cold shock treatment of eggs for 1h, 5min after the insemination. All of the treated eggs examined so far were triploidy. The chromosome number of the triploids was 72, and their karyotype consisted of a diploid set of the maternal species and a haploid set of the paternal species. When silver staining was applied, only NORs from the maternal species (R. o. ocellatus) were stained deeply; no NOR from the paternal species was detected. The aseptic breeding and the cell culture method with larvae newly induced in this study were very useful for facilitating breeding and chromosome studies.