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Effect of Ferrous Ion on Amino Acid Metabolism in Mildiomycin Production by Streptoverticillium rimofaeiens.
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- KISHIMOTO KATSUMITSU
- Technology Department, Hikari Branch Lab., Takeda Chemical Industries, Ltd.
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- PARK YONG SOO
- Biochemical Eng. Lab., Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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- OKABE MITSUYASU
- Biochemical Eng. Lab., Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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- AKIYAMA SHUN-ICHI
- Technology Department, Hikari Branch Lab., Takeda Chemical Industries, Ltd.
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Description
The physiological features of the mildiomycin production by Streptoverticillium rimofaciens were examined in iron-sufficient and -deficient media. Activities of NADP-linked glutamate dehydrogenase (GDH) and aspartate aminotransferase (AAT) were markedly enhanced by the addition of 10 μg/ml of ferrous ion into culture. Ammonium nitrogen assimilation increased with the increase in mildiomycin production. These indicate that ferrous ion contributes the supply of amino acids as a precursor of mildiomycin production. In the iron-sufficient medium, glutamate, aspartate, serine and arginine in cells were 2 to 10-fold to those in the iron-deficient medium. The major amino acid excreted from cells was arginine in the iron-sufficient culture, while in the iron-deficient culture, valine.<br> Change in the amino acid profile by addition of ferrous ion was useful for mildiomycin biosynthesis, in which ferrous ion played a leading role in amino acid metabolism.
Journal
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- The Journal of Antibiotics
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The Journal of Antibiotics 50 (3), 206-211, 1997
JAPAN ANTIBIOTICS RESEARCH ASSOCIATION