A method for separating commercial colistin complex into new components: colistins pro-A, pro-B and pro-C.
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- KIMURA YUKIO
- Faculty of Pharmaceutical Sciences, Mukogawa Women's University
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- KITAMURA HISAMI
- Faculty of Pharmaceutical Sciences, Mukogawa Women's University
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- HAYASHI KYOZO
- Faculty of Pharmaceutical Sciences, Kyoto University
Bibliographic Information
- Other Title
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- Method for Separating Commercial Colist
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Abstract
Commercial colistin was separated into three major components as well as lipid components by reversed phase adsorption chromatography on Diaion HP-20 AG, a macroreticular styrene-divinylbenzene copolymer, without any inorganic salts or detergents, in aqueousorganic solvent as mobile phase. These expected components were colistins A, B and C; there were, however, appreciable differences between these components and colistins A, B and C, isolated by countercurrent distribution. The newly isolated components showed slightly higher potency than colistins A, B and C; their molecular weights, as determined by gel permeation chromatography of 2, 4-dinitrophenyl derivatives on TSK-GEL G2000H (mobile phase: dimethylformamide), were also slightly higher. Accordingly, they were tentatively named colistins pro-A, pro-B and pro-C. During purification by countercurrent distribution (solvent system: sec-BuOH-n-BuOH-0.1 N HCl, 30: 6: 40), colistin pro-A was converted to colistin A. Similarly, colistin pro-B was converted to colistin B, and colistin pro-C to colistin C. Therefore, we concluded that colistins A, B and C are artifacts.
Journal
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- The Journal of Antibiotics
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The Journal of Antibiotics 35 (11), 1513-1520, 1982
JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
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Details 詳細情報について
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- CRID
- 1390282679127235712
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- NII Article ID
- 130003500166
- 40005315268
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- NII Book ID
- AA0069330X
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- COI
- 1:CAS:528:DyaL3sXhtVGlt7Y%3D
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- ISSN
- 18811469
- 00218820
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- NDL BIB ID
- 2617101
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- PubMed
- 7161191
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed