The fermentation, isolation and characterization of a macromolecular peptide antibiotic: An-1.
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- MIYASHIRO SHIGEYOSHI
- Central Research Laboratories, Ajinomoto Co.
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- KIDA TAKAO
- Central Research Laboratories, Ajinomoto Co.
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- SHIBAI HIROSHIRO
- Central Research Laboratories, Ajinomoto Co.
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- SHIIO TSUYOSHI
- Central Research Laboratories, Ajinomoto Co.
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- UDAKA SHIGEZO
- Faculty of Agriculture, Nagoya University
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説明
A new macromolecular peptide antibiotic, named AN-1, was isolated from the culture broth of Streptomyces albus AJ9003. From 18 liters of culture broth (110 units/ml activity) a 300 mg sample of AN-1 was obtained with a specific activity of 1, 160 units/mg was obtained. AN-1 is a basic polypeptide with a molecular weight of 12, 000, isoelectric point of pH 8.3, and gives a single band on SDS polyacrylamide gel electrophoresis. It is soluble in water but insoluble in ethanol, butanol and acetone. It was stable at pH 6-9 but very unstable at pH 2. The UV absorption spectrum shows a maximum at 280 nm. AN-1 had no antibacterial activity against the Gram-positive and Gram-negative bacteria tested, but shows strong inhibitory activity toward Escherichia coli MP2, a macromolecule permeable mutant. In addition to being highly mutagenic, AN-1 inhibits the in vitro cell growth of L1210 (ED50 0.41μg/ml). However, AN-1 had no antitumor activity against mouse leukemia L1210 or Lewis lung carcinoma in mouse.
収録刊行物
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- The Journal of Antibiotics
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The Journal of Antibiotics 37 (1), 27-32, 1984
公益財団法人 日本感染症医薬品協会