The Measurement of 45Ca Using "Ready Cap" Solid Scintillator Developed for Utilization in the Liquid Scintillation Counter.

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  • MUROTA Kaeko
    Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
  • HITOMI Yoshitaka
    Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
  • KAWADA Teruo
    Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
  • FUSHIKI Tohru
    Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
  • KITAJIMA Sakihito
    Radioisotope Research Center, Kyoto University
  • KATO Takahisa
    Radioisotope Research Center, Kyoto University

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Other Title
  • 液体シンチレーションカウンタ用固体シンチレータを用いた｀４５´Ｃａの計測

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Description

We studied the performance of“Ready Cap”solid scintillator developed for the liquid scintillation counter, by using 45Ca which is usually employed to deduce intracellular signaling by the tracer method. The liquid scintillators“AQUASOL-2”and“ATOMLIGHT”were used for comparison. Our findings were as follows: (1) A high counting efficiency of over 90% was achieved with the wide range of 200 to 106 dpm, (2) measurement errors due to the uneven resolution of the solid scintillator and/or due to contents such as biological high polymers causing a hindrance in scintillation process were revealed to be low at below 5.3%, and (3) minimal effects were observed on (1) and (2) above even when actual samples containing biological high polymers and cell extracts with biopolymers detergent agent were used. We conclude that the solid scintillator studied here is practically enough useful for liquid scintillation measurement required in biological experiments.

Journal

  • RADIOISOTOPES

    RADIOISOTOPES 47 (3), 216-221, 1998

    Japan Radioisotope Association

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