Assessment of Measuring Serum Tobramycin Concentration by Radioimmunoassay for the Purpose of Therapeutic Drug Monitoring and Comparison with Other Methods
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- TAKAHASHI Satoru
- The Third Department of Internal Medicine St. Marianna University School of Medicine
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- SASAKI Yasuhito
- Department of Pharmacy St. Marianna University School of Medicine
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- SHINOZAKI Kimikazu
- Department of Radiology and Nuclear Medicine St. Marianna University School of Medicine
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- YAMADA Yukio
- The Third Department of Internal Medicine St. Marianna University School of Medicine
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- SAKAKI Tokuichi
- Department of Radiology, Toho University School of Medicine
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- SANO Takashi
- The Third Department of Internal Medicine St. Marianna University School of Medicine
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- SOMEYA Kazuhiko
- The Third Department of Internal Medicine St. Marianna University School of Medicine
Bibliographic Information
- Other Title
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- 臨床薬物動態論に基づく投与設計を目的としたラジオイムノアッセイ法による血中トブラマイシン測定法の評価と他法との比較
Description
With the purpose to use for therapeutic drug monitoring (TDM), blood concentrations of tobramycin (TOB) in each patient were measured by radioimmunoassay (RIA) . A RIA kit of TOB (Clinical assay-Japan Travenol) was evaluated for precision and recovery, in that partial improvement of the method was made, in order to measure low level of TOB. The RIA was compared with high-performance-liquid-chromatography (HPLC), bioassay (BA) and 2 kinds of enzyme immunoassay (EIA) (EMIT and SLFIA) . The RIA of TOB revealed high precision (1.8-2.4% in C.V.) and high reproducibility (5.0-6.9% in C.V.) . It was found that this RIA kit can be used for measuring low level of serum TOB concentrations by a modification of the method. The total range of measurable blood level is from 0.1 to 16.0μg/ml. The nearly one to one correspondence was observed between RIA and other 4 methods, when 154 samples obtained from 18 cases were measured. A representative case of TDM for TOB was demonstrated, in which predicted concentrations agreed fairly well with actual measured values at steady state. It was concluded that the RIA kit is useful for clinical application of TDM for the adequate dosage regimen of TOB. Modification of the method for rapid assay of a small number of samples will increase the clinical usefulness.
Journal
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- RADIOISOTOPES
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RADIOISOTOPES 32 (6), 280-285, 1983
Japan Radioisotope Association
