ISOLATION OF A NEW WATER-SOLUBLE BASIC ANTIBIOTIC A-396-I

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Two water-soluble basic antibiotics A-396-I and II were isolated from a culture broth of Streptoverticillium eurocidicus A-396. Both antibiotics gave similar analytical data indicative of a molecular formula C19±1H35±2013N3, but different optical activities : A-396-I, [α]D +11.6° (in water), and A-396-II, [α]D +21.8° (in water). A comparison with known antibiotics using thin-layer chromatography suggested that A-396-I is a new antibiotic and A-396-II is identical with hygromycin B.

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