Rapid estimation of the duration of .GAMMA.-ray-induced proliferation delay in human lymphocytes by the harlequin staining technique.

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  • MORIMOTO Kanehisa
    Department of Public Health, Faculty of Medicine, University of Tokyo
  • MIURA Kunihiko
    Department of Public Health, Faculty of Medicine, University of Tokyo
  • KOIZUMI Akira
    Department of Public Health, Faculty of Medicine, University of Tokyo

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Other Title
  • 姉妹染色分体分染法を応用したヒト末梢リンパ球におけるγ線誘発細胞分裂増殖遅延時間の測定
  • 姉妹染色分体分染法を応用したヒト末梢リンパ球におけるγ線誘発細胞分裂増殖遅延時間の測定〔英文〕
  • シマイ センショク ブンタイ ブンセンホウ オ オウヨウシタ ヒト マッショウ

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Abstract

The duration of r-ray-induced proliferation delay in human lymphocyte cultures was measured by a rapid and simple method using fluorescence plus-Giemsa (harlequin) staining to distinguish between cells that had divided different numbers of times in culture. By this rapid method, irradiation of unstimulated (Go) or stimulated (G1) lymphocytes was shown to result in a proliferation delay of 1.2 h/Gy (1.2 h/100 rad) or 1.4 h/Gy (1.4 h/100 rad), respectively, in 64 h cultures: The duration of delays had a linear relationship with doses from 2 to 8 Gy (200 to 800 rad) .

Journal

  • RADIOISOTOPES

    RADIOISOTOPES 33 (1), 21-25, 1984

    Japan Radioisotope Association

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