Double-Tracer Radiography with Sulfur-35 and Phosphorus-32 Using Imaging Plates in Brassica napus L.

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  • ISHIBASHI Hiroki
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • YAMAWAKI Masato
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • HIROSE Atsushi
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • NODA Akihiko
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • KANNO Satomi
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • SAITO Takayuki
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • MASUDA Sayaka
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • SEYAMA Syohei
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • TANOI Keitaro
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo Biotechnology Research Center, The University of Tokyo
  • NAKANISHI Tomoko M.
    Laboratory of Radioisotope Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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Other Title
  • イメージングプレートを用いたナタネにおける<SUP>35</SUP>Sおよび<SUP>32</SUP>Pのダブルトレーサラジオグラフィ

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Description

We present double-tracer autoradiography with 35S and 32P using imaging plates and an aluminum plate to visualize the individual distribution of sulfur and phosphorus in Brassica napus L.. Because of the different attenuation efficiencies of β-rays emitted from 35S and 32P, the specific image of 32P was taken when an aluminum plate was placed between the sample and the imaging plate. The specific image of 35S was obtained by subtracting the 32P image from the 35S and 32P image. It was observed that high amount of 35S was distributed at the edge of the leaves and in root tips. The 32P was distributed throughout the whole plant and it was found that the phosphorous amount was higher at the shoot apical meristems than those in leaves.

Journal

  • RADIOISOTOPES

    RADIOISOTOPES 59 (2), 75-79, 2010

    Japan Radioisotope Association

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