Autoradiography of Lyophilized Animal Sections: Bone Density Measurement in Osteoporosis Model Rats
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- FUJINO Hideki
- Graduate School of Advanced Science and Engineering, Waseda University
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- ASAHARA Nobumitsu
- Tsukuba Laboratories, Nemoto Science Co. Ltd.
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- KIMATA Hideki
- Tokyo New Drug Research Laboratories, KOWA Co. Ltd.
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- TATEISHI Mitsuru
- Tsukuba Laboratories, Nemoto Science Co. Ltd.
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- KOIDE Takashi
- Tokyo New Drug Research Laboratories, KOWA Co. Ltd.
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- BABA Shigeo
- Tsukuba Laboratories, Nemoto Science Co. Ltd. Tokyo University of Pharmacy and Life Science
Bibliographic Information
- Other Title
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- オートラジオグラフィによる骨粗鬆症モデルラットの骨密度測定
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Description
To gain a better understanding on the β-ray radiography of lyophilized animal sections, the bone densities of the osteoporosis rats were measured using a 147Pm planar radiation source. An imaging plate as a radiation sensor was overlaid on the animal section together with a density calibrator. After exposure, radioactivity recorded on the sensor was quantified using a bio-imaging analyzer. The density calibration curve showed linearity in the wide range with a good correlation coefficient(R2≥0.999). The inter- and intra-plate variability showed CV values less than 3.7%. On the measurement of bone density, bone density differences between the sham group and ovariectomized(OVX)group were statistically significant in the femoral cortical(p=0.001)and trabecular bone(p=0.07), and vertebral trabecular bone(p=0.043). Based on these results, we developed a new and valuable method, which made it possible to measure bone density of axial skeleton of a rat, as an alternative to commonly used methods.
Journal
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- RADIOISOTOPES
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RADIOISOTOPES 57 (2), 111-119, 2008
Japan Radioisotope Association
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Details 詳細情報について
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- CRID
- 1390282679132273024
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- NII Article ID
- 130004511478
- 10021071329
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- NII Book ID
- AN00351589
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- ISSN
- 18844111
- 00338303
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- NDL BIB ID
- 9386074
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed