Method of Assessment for 3D Reconstructed Bioartificial Liver Using 13C-glucose Breath Test

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  • ｀１３´Ｃグルコース呼気試験を利用した３次元還流培養系の活性測定とその応用
  • 13Cグルコース呼気試験を利用した3次元還流培養系の活性測定とその応用
  • 13Cグルコース コキ シケン オ リヨウ シタ 3ジゲン カンリュウ バイヨウケイ ノ カッセイ ソクテイ ト ソノ オウヨウ

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Abstract

13C-glucose is consisted of carbon 13, stable isotope. Six carbons in glucose were converted to 13C. 13C-glucose breath test (13C-GBT) is useful for evaluating the cell viability and human metabolic function. It is assessed by 13CO2, metabolite of 13C-glucose, in exhausted gas samples. It is quickly measured by using an isotope ratio mass spectrometer (POC one, Otsuka Pharmaceutical Co. Ltd, Tokyo).<BR>In the study we made, we have assessed glucose metabolism in the reconstructed liver organoid using by 13C-GBT. We made a 3-dimentional reconstructed organoid (mini artificial liver model) using a radial-flow bioreactor(RFB), and evaluated viability of the organoid by 13C-GBT. The liver organoid was constructed by co-culture with immortalized mouse hepatocytes, sinusoidal endothelial cells and hepatic stellate cells. Exhaust gas was sampled in gas bags from the closed circuit of RFB system.<BR>Firstly we observed transition of 13CO2 in exhaust gas along culture time. Glucose metabolism was enhanced by not only hepatocyte growth, but co-culture of non-parenchymal cells. We also examined ischemia/reperfusion injury model in the reconstructed liver organoid. 13C-GBT clearly showed depressed metabolism by ischemic effect to the liver organoid.<BR>In conclusion, 13C-GBT is extremely useful for assessment of viability and glucose metabolism in real-time, easily and high sensitivity not only in vivo but in vitro.

Journal

  • RADIOISOTOPES

    RADIOISOTOPES 59 (7), 435-440, 2010

    Japan Radioisotope Association

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